The Role of Mannose-Binding Lectin-2 Gene Polymorphisms in Patients with Colorectal Cancer

Purpose: The mannose-binding lectin pathway of innate immunity is part of the first line of defense against microorganisms. Variations in mannose-binding lectin levels or activity are caused by single-nucleotide polymorphisms in the promoter region. Mannosebinding lectin may play a role as a phase reactant due to inflammatory processes related to cancer disease. Such inflammatory processes are well known in colorectal cancer and have been shown to be independent of stage of disease. We aimed to investigate whether profile of mannose-binding lectin-2 -221G˃C genotyping may be associated with the risk of colorectal cancer. Materials and Methods: The study group consisted of 107 patients were diagnosed with histologically confirmed cancer of the colon or rectum. The control group consisted of 99 were selected among healthy people. Genomic DNA of control and patients was extracted from whole blood using High Pure PCR template preparation kit. Genotyping of mannose-binding lectin-2 polymorphisms were detected by using a mannose-binding lectin-2 mutation detection kit in real-time PCR. Chi-square or Fisher’s Exact Tests were used to evaluate the distribution of the -221G˃C genotypes among the patients and control subjects. Associations between -221G˃C genotype and colorectal cancer risk were analyzed by binary logistic regression. Results: Logistic regression analyses showed that the mannose-binding lectin-2 GC genotype was associated with a significantly increased risk of colorectal cancer. The odds ratio of colorectal cancer for the mannose-binding lectin-2 GC genotype was 2.095 (95% CI=1.76-3.731, P=0.012) compared with the control group. Conclusion: These results suggest that mannose-binding lectin-2 G/C allele gene polymorphisms may be associated with genetic susceptibility of colorectal cancer.